Enhanced Vascular Endothelial Growth Factor Gene Expression in Ischaemic Skin of Critical Limb Ischaemia Patients | Zendy

Silvia Bleda | Zendy; Joaquín De Haro | Zendy; Francisco Acín | Zendy; C Varela | Zendy; Leticia Esparza | Zendy

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Enhanced Vascular Endothelial Growth Factor Gene Expression in Ischaemic Skin of Critical Limb Ischaemia Patients

Author(s) -

Silvia Bleda,

Joaquín De Haro,

Francisco Acín,

C Varela,

Leticia Esparza

Publication year - 2012

Publication title -

international journal of vascular medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.411

H-Index - 27

eISSN - 2090-2832

pISSN - 2090-2824

DOI - 10.1155/2012/691528

Subject(s) - vascular endothelial growth factor , medicine , pathogenesis , vegf receptors , ischemia , gene expression , hypoxia (environmental) , real time polymerase chain reaction , downregulation and upregulation , reverse transcription polymerase chain reaction , gene , pathology , biology , biochemistry , chemistry , organic chemistry , oxygen

Objectives . To perform a quantitative analysis of the vascular endothelial growth factor (VEGF) gene transcription in the skin of ischemic legs and provide information for VEGF in the pathogenesis in critical limb ischemia (CLI). Methods . Skin biopsies were obtained from 40 patients with CLI. Control samples came from 44 patients with chronic venous disease. VEGF gene expression was analysed using quantitative polymerase chain reaction. Results . Patients with CLI had higher skin VEGF expression than control group (RQ: 1.3 ± 0.1 versus 1, P = 0.04). Conclusions . We found an association between ischemic skin and an elevated VEGF expression in legs from patients with CLI. These data support that the mechanism for VEGF upregulation in hypoxia conditions is intact and acts appropriately in the ischaemic limbs from patients with CLI.

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