RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma | Zendy

Shravan K Bankey | Zendy; Ganesh G. Tapadiya | Zendy; Jasvant Lamale | Zendy; Deepti Jain | Zendy; Shweta Saboo | Zendy; S. S. Khadabadi | Zendy

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RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma

Author(s) -

Shravan K Bankey,

Ganesh G. Tapadiya,

Jasvant Lamale,

Deepti Jain,

Shweta Saboo,

S. S. Khadabadi

Publication year - 2012

Publication title -

journal of analytical methods in chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.407

H-Index - 25

eISSN - 2090-8865

pISSN - 2090-8873

DOI - 10.1155/2012/625979

Subject(s) - rimonabant , chromatography , human plasma , high performance liquid chromatography , chemistry , biochemistry , receptor , cannabinoid receptor , agonist

A simple, accurate, and precise HPLC method was developed and validated for determination of rimonabant in human plasma. Following liquid-liquid extraction, chromatographic separation was accomplished using C18 column with mobile phase consisting of acetonitrile : water (90 : 10, v/v), drug was detected at 260 nm using UVdetector. The LOD and LOQ were 3.0 and 10.0 μ g/L, respectively. The method is linear in the interval 50.0–1000.0 μ g/L. The average extraction recovery of drug from plasma was found to be 92.2%. The percent CV of the method was found to be less than 10.8%, and accuracy was found between 94.5 and 106.7%. The assay may be applied to a pharmacokinetic and bioequivalence study of rimonabant.

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