Protective Effects of Peroxisome Proliferator-Activated Receptor-αAgonist, Wy14643, on Hypoxia/Reoxygenation Injury in Primary Rat Hepatocytes | Zendy

Ke Chen | Zendy; Yuanhai Li | Zendy; Siqi Xu | Zendy; Shenghong Hu | Zendy; Lei Zhang | Zendy

Open Access

Protective Effects of Peroxisome Proliferator-Activated Receptor-αAgonist, Wy14643, on Hypoxia/Reoxygenation Injury in Primary Rat Hepatocytes

Author(s) -

Ke Chen,

Yuanhai Li,

Siqi Xu,

Shenghong Hu,

Lei Zhang

Publication year - 2011

Publication title -

ppar research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.164

H-Index - 49

eISSN - 1687-4765

pISSN - 1687-4757

DOI - 10.1155/2012/547980

Subject(s) - agonist , hypoxia (environmental) , peroxisome proliferator activated receptor , receptor , oxidative stress , hepatocyte , downregulation and upregulation , chemistry , glutathione , pharmacology , medicine , endocrinology , biochemistry , enzyme , in vitro , gene , oxygen , organic chemistry

This study investigates the effects and possible mechanism of an agonist of PPAR α , Wy14643, on primary hepatocytes subjected to H/R injury in rats. H/R induced a significant increase ALT, AST, MDA in the culture medium and ROS in the hepatocytes. These effects were reversed by pretreatment with Wy14643 in the dose-dependent manner. The activity of SOD and the level of GSH in the hepatocytes were decreased after H/R, which were increased by Wy14643 pretreatment. Moreover, the mRNA expressions of PPAR α significantly increased in H/R+Wy14643 groups when compared with that in H/R group. A PPAR α agonist, Wy14643, exerts significant protective effect against H/R injury in primary hepatocytes via PPAR α activation and attenuating oxidative stress.

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