Isoliquiritigenin-Induced Differentiation in Mouse Melanoma B16F0 Cell Line | Zendy

Xiaoyu Chen | Zendy; Bo Zhang | Zendy; Xuan Yuan | Zendy; Fan Yang | Zendy; Jinglei Liu | Zendy; Hong Zhao | Zendy; Liangliang Liu | Zendy; Yanming Wang | Zendy; Zhenhua Wang | Zendy; Qiusheng Zheng | Zendy

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Isoliquiritigenin-Induced Differentiation in Mouse Melanoma B16F0 Cell Line

Author(s) -

Xiaoyu Chen,

Bo Zhang,

Xuan Yuan,

Fan Yang,

Jinglei Liu,

Hong Zhao,

Liangliang Liu,

Yanming Wang,

Zhenhua Wang,

Qiusheng Zheng

Publication year - 2012

Publication title -

oxidative medicine and cellular longevity

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.494

H-Index - 93

eISSN - 1942-0900

pISSN - 1942-0994

DOI - 10.1155/2012/534934

Subject(s) - isoliquiritigenin , liquiritigenin , reactive oxygen species , in vitro , melanoma , cell culture , in vivo , biology , flavonoid , cell growth , cellular differentiation , cancer research , chemistry , pharmacology , biochemistry , antioxidant , medicine , gene , genetics , pathology , alternative medicine

The chemotherapeutical treatment is very limited for malignant melanoma, a highly lethal disease occurs globally. Natural products derived from traditional Chinese medicine licorice are attractive in quest new treatments due to their anti-tumor activities. A new dietary flavonoid isoliquiritigenin (ISL) were thus investigated to indentify its anti-melanoma activities on mouse melanoma B16F0 cells in present study. Using biochemical and free radical biological experiments in vitro, we identified the pro-differentiated profiles of ISL and evaluated the role of reactive oxygen species (ROS) during B16F0 cell differentiation. The data showed a strong dose-response relationship between ISL exposure and the characteristics of B16F0 differentiation in terms of morphology changes and melanogenesis. The accumulated intercellular ROS during exposure are necessary to support ISL-induced differentiation, which was proven by additional redox modulators. It was confirmed further by the relative activities of enzymes and genes modulated melanogenesis in ISL-treatments with or without ROS modulators. The tumorigenicity of ISL-treated cells was limited significantly by using the colony formation assay in vitro and an animal model assay in vivo respectively. Our research demonstrated that isoliquiritigenin is a differentiation-inducing agent, and its mechanisms involve ROS accumulation facilitating melanogenesis

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