English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Mymensingh is the most endemic district for kala-azar in Bangladesh.  Phlebotomus argentipes  remains the only known vector although a number of sand fly species are prevalent in this area. Genotyping of sand flies distributed in a VL endemic area was developed by a PCR and restriction-fragment-length polymorphism (RFLP) of 18S rRNA gene of sand fly species. Using the RFLP-PCR analysis with  Afa I and  Hin fI restriction enzymes,  P. argentipes, P. papatasi , and  Sergentomyia  species could be identified. Among 1,055 female sand flies successfully analyzed for the species identification individually, 64.4% flies was classified as  Sergentomyia  species, whereas 35.6% was identified as  P. argentipes  and no  P. papatasi  was found. Although infection of  Leishmania  within the sand flies was individually examined targeting leishmanial minicircle DNA, none of the 1,055 sand flies examined were positive for  Leishmania  infection. The RFLP-PCR could be useful tools for taxonomic identification and  Leishmania  infection monitoring in endemic areas of Bangladesh.

Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh