Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility | Zendy

Martina Schleicher | Zendy; Jan Hansmann | Zendy; Bentsian Elkin | Zendy; Petra J. Kluger | Zendy; Simone Liebscher | Zendy; Agnes J. Huber | Zendy; Olaf Fritze | Zendy; Christine Schille | Zendy; Michaela Müller | Zendy; Katja SchenkeLayland | Zendy; Martina Seifert | Zendy; Heike Walles | Zendy; Hans-Peter Wendel | Zendy; Ulrich A. Stock | Zendy

Open Access

Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility

Author(s) -

Martina Schleicher,

Jan Hansmann,

Bentsian Elkin,

Petra J. Kluger,

Simone Liebscher,

Agnes J. Huber,

Olaf Fritze,

Christine Schille,

Michaela Müller,

Katja SchenkeLayland,

Martina Seifert,

Heike Walles,

Hans-Peter Wendel,

Ulrich A. Stock

Publication year - 2012

Publication title -

international journal of biomaterials

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.576

H-Index - 28

eISSN - 1687-8795

pISSN - 1687-8787

DOI - 10.1155/2012/397813

Subject(s) - oligonucleotide , thrombogenicity , materials science , in vivo , biomedical engineering , endothelial progenitor cell , protein adsorption , coating , chemistry , endothelial stem cell , dna , nanotechnology , in vitro , composite material , polymer , biochemistry , immunology , medicine , biology , platelet , microbiology and biotechnology

In vivo self-endothelialization by endothelial cell adhesion on cardiovascular implants is highly desirable. DNA-oligonucleotides are an intriguing coating material with nonimmunogenic characteristics and the feasibility of easy and rapid chemical fabrication. The objective of this study was the creation of cell adhesive DNA-oligonucleotide coatings on vascular implant surfaces. DNA-oligonucleotides immobilized by adsorption on parylene (poly(monoaminomethyl-para-xylene)) coated polystyrene and ePTFE were resistant to high shear stress (9.5 N/m 2 ) and human blood serum for up to 96 h. Adhesion of murine endothelial progenitor cells, HUVECs and endothelial cells from human adult saphenous veins as well as viability over a period of 14 days of HUVECs on oligonucleotide coated samples under dynamic culture conditions was significantly enhanced ( P < 0.05). Oligonucleotide-coated surfaces revealed low thrombogenicity and excellent hemocompatibility after incubation with human blood. These properties suggest the suitability of immobilization of DNA-oligonucleotides for biofunctionalization of blood vessel substitutes for improved in vivo endothelialization.

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