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Studies on Acetone Powder and Purified Rhus Laccase Immobilized on Zirconium Chloride for Oxidation of Phenols
Author(s) -
Rong Lü,
Tetsuo Miyakoshi
Publication year - 2012
Publication title -
enzyme research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.439
H-Index - 39
eISSN - 2090-0406
pISSN - 2090-0414
DOI - 10.1155/2012/375309
Subject(s) - laccase , chemistry , acetone , phenols , chloride , ammonium chloride , zirconium , thermostability , nuclear chemistry , guaiacol , chromatography , organic chemistry , catalysis , enzyme
Rhus laccase was isolated and purified from acetone powder obtained from the exudates of Chinese lacquer trees ( Rhus vernicifera ) from the Jianshi region, Hubei province of China. There are two blue bands appearing on CM-sephadex C-50 chromatography column, and each band corresponding to Rhus laccase 1 and 2, the former being the major constituent, and each had an average molecular weight of approximately 110 kDa. The purified and crude Rhus laccases were immobilized on zirconium chloride in ammonium chloride solution, and the kinetic properties of free and immobilized Rhus laccase, such as activity, molecular weight, optimum pH, and thermostability, were examined. In addition, the behaviors on catalytic oxidation of phenols also were conducted.

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