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Rhus  laccase was isolated and purified from acetone powder obtained from the exudates of Chinese lacquer trees ( Rhus vernicifera ) from the Jianshi region, Hubei province of China. There are two blue bands appearing on CM-sephadex C-50 chromatography column, and each band corresponding to  Rhus  laccase 1 and 2, the former being the major constituent, and each had an average molecular weight of approximately 110 kDa. The purified and crude  Rhus  laccases were immobilized on zirconium chloride in ammonium chloride solution, and the kinetic properties of free and immobilized  Rhus  laccase, such as activity, molecular weight, optimum pH, and thermostability, were examined. In addition, the behaviors on catalytic oxidation of phenols also were conducted.

Studies on Acetone Powder and Purified Rhus Laccase Immobilized on Zirconium Chloride for Oxidation of Phenols