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Background . In gastric carcinogenesis, changes of DNA methylation appear to be an early molecular event, and the genome-wide methylation state is closely correlated with the level of long interspersed nucleotide element-1 (LINE-1) methylation. In this study, we measured LINE-1 methylation level according to genetic instability and evaluated the effect of  Helicobacter pylori  infection on genetic instability in gastric epithelial dysplasia.  Methods . Total 100 tissue samples of gastric epithelial dysplasia were analyzed. Seven loci that linked to tumor suppressor genes were used to identify significant structural chromosomal aberrations. Microsatellite status was investigated for two different microsatellite marker loci (BAT25 and BAT26). Also, we measured LINE-1 methylation level by combined bisulfite restriction analysis (COBRA-LINE-1) method.  Results . There were no significant differences of LINE-1 methylation level according to chromosomal/microsatellite instability and  H. pylori  state. In the dysplastic lesions with  H. pylori  infection, LINE-1 methylation level of MSI lesion was significantly lower than that of microsatellite stable (MSS) lesion (40.23 ± 4.47 versus 43.90 ± 4.81%,  P  < 0.01).  Conclusions . In gastric epithelial dysplasia with  H. pylori  infection, MSI is correlated with reduced LINE-1 methylation level. Coexistence of  H. pylori  infection and MSI might be a driving force of gastric carcinogenesis.

Association between Genetic Instability andHelicobacter pyloriInfection in Gastric Epithelial Dysplasia