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Objective . To determine the prevalence and the genotypes of  Enterocytozoon bieneusi  in stool specimens from HIV patients.   Methods . This cross-sectional study was carried out in Kinshasa hospitals between 2009 and 2012. Detection of microsporidia including  E. bieneusi  and  E. intestinalis  was performed in 242 HIV-infected patients. Typing was based on DNA polymorphism of the ribosomal DNA ITS region of  E. bieneusi . PCRRFLP generated with two restriction enzymes (Nla III and Fnu 4HI) in PCR-amplified ITS products for classifying strains into different lineages. The diagnosis performance of the indirect immune-fluorescence-monoclonal antibody (IFI-AcM) was defined in comparison with real-time PCR as the gold standard.  Results . Out of 242 HIV-infected patients, using the real-time PCR, the prevalence of  E. bieneusi  was 7.9% ( n  = 19) among the 19  E. bieneusi , one was coinfected with  E. intestinalis . In 19  E. bieneusi  persons using PCR-RFLP method, 5 type I strains of  E. bieneusi  (26.3%) and 5 type IV strains of  E. bieneusi  (26.3%) were identified. The sensitivity of IFI-AcM was poor as estimated 42.1%.   Conclusion . Despite different PCR methods, there is possible association between HIVinfection, geographic location (France, Cameroun, Democratic Republic of Congo), and the concurrence of type I and type IV strains.

journal of parasitology research

<i>Enterocytozoon bieneusi</i>Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo

Enterocytozoon bieneusiIdentification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo