Environmental Factors Preceding A40 Monomer to Oligomers and the Detection of Oligomers in Alzheimer's Disease Patient Serum

We present here environmental factors including pH shifts, temperature, and metal ions surrounding A β 40 monomer to precede the oligomers. We also suggest a new idea to detect A β 40 oligomers with anti-A β 40 monoclonal antibody using enzyme-linked immunosorbent assay. This method involves the different sensitivity of the thermal shifts between A β 40 monomer and the oligomers. The idea is useful for the diagnostics of Alzheimer's disease to detect A β 40 oligomers in the serum from the patients.