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A micropropagation protocol was developed which may assist in the safeguarding and augmentation of dwindling natural populations of  Clinopodium odorum  (Griseb.) Harley, a critically and endangered medicinal plant. Factors affecting culture initiation bud sprouting and growth, rooting, and acclimatization were studied, using nodal segments of  in vitro  germinated seedling as primary explants on six media supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP) (0.5–1.5 and 2-Naphthalene acetic acid (NAA) (0.5–1.5). Best results for culture initiation with sustainable multiplication rates (100%) were obtained on WP medium without any growth regulator. WP with the addition of 0.5 : 1 or 0.5 : 1.5) of BAP and NAA promoted a higher elongation; however, the optimum number of nodes were obtained in plantlets grown on 1/2 MS with the addition of 1 : 1.5 of BAP and NAA. Culture of sectioned individual nodes transferred to the media with different rates of BAP and NAA 1/2 MS-9 (1.5 : 1.5), SH-8 (1.5 : 1.0), and 1/2 B5-4 (1.0 : 0.5) media resulted in no proliferated shoots. The  in vitro  plants were successfully acclimatized garden soil and sand (2 : 1) in the greenhouse, with over 90% survival rate. The  in vitro -grown plants could be transferred to  ex vitro  conditions and the efficacy in supporting  ex vitro  growth was assessed, with a view to develope longer-term strategies for the transfer and reintroduction into natural habitats.

In Vitro Propagation of Muña-Muña (Clinopodium odorum (Griseb.) Harley)