Intensification of Doxorubicin-Related Oxidative Stress in the Heart by Hypothyroidism Is Not Related to the Expression of Cytochrome P450 NADPH-Reductase and Inducible Nitric Oxide Synthase, As Well As Activity of Xanthine Oxidase | Zendy

Jarosław Dudka | Zendy; Franciszek Burdan | Zendy; Agnieszka Korga-Plewko | Zendy; Magdalena Iwan | Zendy; B Madej | Zendy; Monika CendrowskaPinkosz | Zendy; Agnieszka KorobowiczMarkiewicz | Zendy; Barbara JodłowskaJędrych | Zendy; W Matysiak | Zendy

Open Access

Intensification of Doxorubicin-Related Oxidative Stress in the Heart by Hypothyroidism Is Not Related to the Expression of Cytochrome P450 NADPH-Reductase and Inducible Nitric Oxide Synthase, As Well As Activity of Xanthine Oxidase

Author(s) -

Jarosław Dudka,

Franciszek Burdan,

Agnieszka Korga-Plewko,

Magdalena Iwan,

B Madej,

Monika CendrowskaPinkosz,

Agnieszka KorobowiczMarkiewicz,

Barbara JodłowskaJędrych,

W Matysiak

Publication year - 2012

Publication title -

oxidative medicine and cellular longevity

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.494

H-Index - 93

eISSN - 1942-0900

pISSN - 1942-0994

DOI - 10.1155/2012/139327

Subject(s) - oxidative stress , xanthine oxidase , doxorubicin , cardiotoxicity , endocrinology , chemistry , pharmacology , medicine , glutathione reductase , nitric oxide synthase , lipid peroxidation , glutathione , reductase , glutathione peroxidase , enzyme , biochemistry , superoxide dismutase , toxicity , chemotherapy

Cytochrome P450 NADPH-reductase (P450R), inducible synthase (iNOS) and xanthine oxidase play an important role in the antracycline-related cardiotoxicity. The expression of P450R and iNOS is regulated by triiodothyronine. The aim of this study was to evaluate the effect of methimazole-induced hypothyreosis on oxidative stress secondary to doxorubicin administration. 48 hours after methimazole giving cessation, rats were exposed to doxorubicin (2.0, 5.0 and 15 mg/kg). Blood and heart were collected 4, 48 and 96 h after the drug administration. Animals exposed exclusively to doxorubicin or untreated ones were also assessed. The hypothyreosis (0.025% of methimazole) significantly increased the doxorubicin effect on the cardiac carbonyl group and they may increase the glutathione level. An insignificant effect of methimazole was noticed in case of the cardiac lipid peroxidation product, the amount of DNA oxidative damages, iNOS and xanthine oxidase-enzymes responsible for red-ox activation of doxorubicin. However, the concentration of P450R was affected by a lower dose of methimazole in rats administered with doxorubicin. Since in rats receiving doxorubicin changes in oxidative stress caused by methimazole were not accompanied by elevation of bioreductive enzymes, it may be concluded that these changes in the oxidative stress were not related to the tested enzymes

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research