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This paper reports the isolation and identification of chitinase-producing  Bacillus  from chitin-containing wastes, production of a thermostable and alkaline chitinasese, and enzyme characterization.  Bacillus thuringiensis  subsp.  kurstaki  HBK-51 was isolated from soil and was identified. Chitinase was obtained from supernatant of  B. thuringiensis  HBK-51 strain and showed its optimum activity at 110°C and at pH 9.0. Following 3 hours of incubation period, the enzyme showed a high level of activity at 110°C (96% remaining activity) and between pH 9.0 and 12.0 (98% remaining activity). Considering these characteristics, the enzyme was described as hyperthermophile-thermostable and highly alkaline. Two bands of the enzyme weighing 50 and 125 kDa were obtained following 12% SDS-PAGE analyses. Among the metal ions and chemicals used, Ni 2+  (32%), K +  (44%), and Cu 2+  (56%) increased the enzyme activity while EDTA (7%), SDS (7%), Hg 2+  (11%), and ethyl-acetimidate (20%) decreased the activity of the enzyme.  Bacillus thuringiensis  subsp.  kurstaki  HBK-51 is an important strain which can be used in several biotechnological applications as a chitinase producer.

Production of a Thermostable and Alkaline Chitinase by Bacillus thuringiensis subsp. kurstaki Strain HBK-51