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The efficacy of extracts and essential oils from  Allium tuberosum, Coriandrum sativum, Cymbopogon martini, Cymbopogon winterianus,  and  Santolina chamaecyparissus  was evaluated against  Candida  spp. isolates from the oral cavity of patients with periodontal disease. The most active oil was fractionated and tested against  C. albicans  biofilm formation. The oils were obtained by water-distillation and the extracts were prepared with macerated dried plant material. The Minimal Inhibitory Concentration—MIC was determined by the microdilution method. Chemical characterization of oil constituents was performed using Gas Chromatography and Mass Spectrometry (GC-MS). C. sativum activity oil upon cell and biofilm morphology was evaluated by Scanning Electron Microscopy (SEM). The best activities against planktonic  Candida  spp. were observed for the essential oil and the grouped F 8–10  fractions from  C. sativum . The crude oil also affected the biofilm formation in  C. albicans  causing a decrease in the biofilm growth. Chemical analysis of the F 8–10  fractions detected as major active compounds, 2-hexen-1-ol, 3-hexen-1-ol and cyclodecane. Standards of these compounds tested grouped provided a stronger activity than the oil suggesting a synergistic action from the major oil constituents. The activity of  C. sativum  oil demonstrates its potential for a new natural antifungal formulation.

Action ofCoriandrum sativumL. Essential Oil upon OralCandida albicansBiofilm Formation