DNA binding and photo-induced DNA cleavage activity of Elsinochrome A in visible light | Zendy

Fei Ma | Zendy; Jing Tang | Zendy; Chao Yang | Zendy; Yuying Feng | Zendy; Shaohua Wei | Zendy; Jian Shen | Zendy; Jiahong Zhou | Zendy; Lin Zhou | Zendy

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DNA binding and photo-induced DNA cleavage activity of Elsinochrome A in visible light

Author(s) -

Fei Ma,

Jing Tang,

Chao Yang,

Yuying Feng,

Shaohua Wei,

Jian Shen,

Jiahong Zhou,

Lin Zhou

Publication year - 2011

Publication title -

spectroscopy an international journal

Language(s) - English

Resource type - Journals

eISSN - 1875-922X

pISSN - 0712-4813

DOI - 10.1155/2011/932594

Subject(s) - ethidium bromide , dna , chemistry , quenching (fluorescence) , fluorescence , biophysics , binding site , cleavage (geology) , photochemistry , microbiology and biotechnology , biochemistry , biology , optics , physics , paleontology , fracture (geology)

The interaction of Elsinochrome A (EA) with calf thymus DNA (CT-DNA) has been investigated by UV-vis spectra and fluorescence spectra. The results show that EA can bind with CT-DNA and binding sites are destroyed after irradiation by visible light, which indicates that EA is a promising candidate for photodynamic therapy. In addition, the binding mechanism is studied using fluorescence quenching test and ethidium bromide (EB) replace assay experiments. The results suggest that EA and CT-DNA are binding with a strong force and the major binding mode of EA with DNA could be the electrostatic binding.

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