Open AccessPerfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung
Author(s) -
Jeffrey A. Kazzaz,
Marlene S. Strayer,
Jichuan Wu,
Daniel J. Malone,
Hshi-chi Koo,
Thomas H. Shaffer,
Jonathan M. Davis,
David S. Strayer,
Marla R. Wolfson
Publication year - 2011
Publication title -
pulmonary medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 30
eISSN - 2090-1836
pISSN - 2090-1844
DOI - 10.1155/2011/918036
Subject(s) - luciferase , saline , gene delivery , genetic enhancement , recombinant dna , microbiology and biotechnology , transgene , gene expression , lung , transduction (biophysics) , suspension culture , a549 cell , medicine , gene , cell culture , biology , biophysics , transfection , biochemistry , genetics
We compared lung delivery methods of recombinant adenovirus (rAd): (1) rAd suspended in saline, (2) rAd suspended in saline followed by a pulse-chase of a perfluorochemical (PFC) liquid mixture, and (3) a PFC-rAd suspension. Cell uptake, distribution, and temporal expression of rAd were examined using A549 cells, a murine model using luciferase bioluminescence, and histological analyses. Relative to saline, a 4X increase in transduction efficiency was observed in A549 cells exposed to PFC-rAd for 2–4 h. rAd transgene expression was improved in alveolar epithelial cells, and the level and distribution of luciferase expression when delivered in PFC-rAd suspensions consistently peaked at 24 h. These results demonstrate that PFC-rAd suspensions improve distribution and enhance rAd-mediated gene expression which has important implications in improving lung function by gene therapy.
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