First Preliminary Report on Isolation and Characterization of NovelAcinetobacterspp. in Casing Soil Used for Cultivation of Button Mushroom,Agaricus bisporus(Lange) Imbach
Author(s) -
Devendra Kumar Choudhary
Publication year - 2011
Publication title -
international journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 40
eISSN - 1687-9198
pISSN - 1687-918X
DOI - 10.1155/2011/790285
Subject(s) - agaricus bisporus , mushroom , casing , compost , acinetobacter , horticulture , manure , biology , proteobacteria , 16s ribosomal rna , microbiology and biotechnology , food science , veterinary medicine , botany , agronomy , bacteria , medicine , antibiotics , engineering , genetics , petroleum engineering
Despite evaluation of large number of agroindustrial wastes for their use as casing material for Agaricus bisporus (Lange) Imbach cultivation, scant attention has been given to the importance of biological properties of casing materials. In the present study, an attempt was made to characterize the bacterial flora in casing layer, namely, Farm Yard Manure (FYM) and Spent Mushroom Substrate/spent compost (SMS/SC) (FYM+SC, 3 : 1) and FYM and Vermi Compost (VC) (FYM+VC, 3 : 1), employing partial 16S rDNA sequencing. Available data showed a significant variety of organisms that included Acinetobacter and Pseudomonas of the γ -proteobacteria, that were the most frequently encountered genera. This is the first preliminary report on the microbial diversity of casing soils and demonstrates the presence of Acinetobacter spp. that has not been previously described in casing material.
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