Nanoparticles of Conjugated Methotrexate-Human Serum Albumin: Preparation and Cytotoxicity Evaluations | Zendy

Azade Taheri | Zendy; Fatemeh Atyabi | Zendy; Faranak Salman Nouri | Zendy; Fatemeh Ahadi | Zendy; Mohammad Ali Derakhshan | Zendy; Mohsen Amini | Zendy; Mohammad Hossein Ghahremani | Zendy; Seyed Nasser Ostad | Zendy; Pooria Mansoori | Zendy; Rassoul Dinarvand | Zendy

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Nanoparticles of Conjugated Methotrexate-Human Serum Albumin: Preparation and Cytotoxicity Evaluations

Author(s) -

Azade Taheri,

Fatemeh Atyabi,

Faranak Salman Nouri,

Fatemeh Ahadi,

Mohammad Ali Derakhshan,

Mohsen Amini,

Mohammad Hossein Ghahremani,

Seyed Nasser Ostad,

Pooria Mansoori,

Rassoul Dinarvand

Publication year - 2010

Publication title -

journal of nanomaterials

Language(s) - English

Resource type - Journals

eISSN - 1687-4129

pISSN - 1687-4110

DOI - 10.1155/2011/768201

Subject(s) - carbodiimide , methotrexate , nanoparticle , conjugate , albumin , human serum albumin , conjugated system , bovine serum albumin , materials science , particle size , differential scanning calorimetry , nuclear chemistry , serum albumin , dynamic light scattering , cytotoxicity , chromatography , chemistry , biochemistry , polymer chemistry , organic chemistry , nanotechnology , in vitro , polymer , immunology , biology , mathematical analysis , thermodynamics , mathematics , physics

Methotrexate-human serum albumin conjugates were developed by a simple carbodiimide reaction. Methotrexate-human serum albumin conjugates were then crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) to form nanoparticles. The size of nanoparticles determined by laser light scattering and TEM was between 90–150 nm. Nanoparticles were very stable at physiologic conditions (PBS pH 7.4, 37∘C) and after incubation with serum. The effect of amount of EDC used for crosslinking on the particle size and free amino groups of nanoparticles was examined. The amount of crosslinker showed no significant effect on the size of nanoparticles but free amino groups of nanoparticles were decreased by increasing the crosslinker. The physicochemical interactions between methotrexate and human serum albumin were investigated by differential scanning calorimetry (DSC). Nanoparticles were more cytotoxic on T47D cells compared to free methotrexate. Moreover, methotrexate-human serum albumin nanoparticles decreased the IC50 value of methotrexate on T47D cells in comparison with free methotrexate

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