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Mine tailing remediation aims to reduce the rate of sulfide mineral oxidation. Earlier studies showed that photosynthetic biofilms may act as a physical barrier against oxygen diffusion. Currently, a long-term assay (6 months) is required to evaluate the solid phase redistribution of the Pb, Fe, Cu, and Zn originally present in historic and calcareous mine tailing samples (in our case from a semiarid region in North-Central Mexico). The presence of biofilms may provide chemical gradients and physical conditions that shift the proportion of Fe, Cu, and Zn originally associated with oxides to carbonates and organic matter/sulfide fractions

Growth of Photosynthetic Biofilms and Fe, Pb, Cu, and Zn Speciation in Unsaturated Columns with Calcareous Mine Tailings from Arid Zones