Antiosteoporotic Activity ofDioscorea alataL. cv. Phyto through Driving Mesenchymal Stem Cells Differentiation for Bone Formation | Zendy

KangYung Peng | Zendy; LinYea Horng | Zendy; HuiChing Sung | Zendy; HuiChuan Huang | Zendy; Rong-Tsun Wu | Zendy

Open Access

Antiosteoporotic Activity ofDioscorea alataL. cv. Phyto through Driving Mesenchymal Stem Cells Differentiation for Bone Formation

Author(s) -

KangYung Peng,

LinYea Horng,

HuiChing Sung,

HuiChuan Huang,

Rong-Tsun Wu

Publication year - 2011

Publication title -

evidence-based complementary and alternative medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.552

H-Index - 90

eISSN - 1741-4288

pISSN - 1741-427X

DOI - 10.1155/2011/712892

Subject(s) - alkaline phosphatase , ovariectomized rat , bone marrow , chemistry , stromal cell , mesenchymal stem cell , bone mineral , osteoporosis , microbiology and biotechnology , endocrinology , medicine , biology , biochemistry , enzyme , hormone

The aim of this study was to evaluate the effect of an ethanol extract of the rhizomes of Dioscorea alata L. cv. Phyto, Dispo85E, on bone formation and to investigate the mechanisms involved. Our results showed that Dispo85E increased the activity of alkaline phosphatase (ALP) and bone nodule formation in primary bone marrow cultures. In addition, Dispo85E stimulated pluripotent C3H10T1/2 stem cells to differentiate into osteoblasts rather than adipocytes. Our in vivo data indicated that Dispo85E promotes osteoblastogenesis by increasing ALP activity and bone nodule formation in both intact and ovariectomized (OVX) mice. Microcomputed tomography ( μ CT) analysis also showed that Dispo85E ameliorates the deterioration of trabecular bone mineral density (tBMD), trabecular bone volume/total volume (BV/TV), and trabecular bone number (Tb.N) in OVX mice. Our results suggested that Dispo85E is a botanical drug with a novel mechanism that drives the lineage-specific differentiation of bone marrow stromal cells and is a candidate drug for osteoporosis therapy.

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