Open AccessHyperplastic Polyps Are Innocuous Lesions in Hereditary Nonpolyposis Colorectal Cancers
Author(s) -
Doug Speake,
Jacintha O’Sullivan,
D. Gareth Evans,
Fiona Lalloo,
James Hill,
R F McMahon
Publication year - 2011
Publication title -
international journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 22
eISSN - 2090-1410
pISSN - 2090-1402
DOI - 10.1155/2011/653163
Subject(s) - microsatellite instability , methylation , immunohistochemistry , dna methylation , hyperplastic polyp , medicine , colorectal cancer , pathology , oncology , cancer research , gastroenterology , biology , microsatellite , genetics , cancer , gene , gene expression , colonoscopy , allele
Aims . To compare methylation profiles, protein expression, and microsatellite instability (MSI) of sporadic, HNPCC, and familial hyperplastic polyps (HPs). Methods . Methylation-specific PCR (MSP) and pyrosequencing assessed p16, MGMT, hMLH-1, MINT 1, and MINT 31 methylation. IHC (Immunohistochemistry) assessed Ki67, CK20, hMLH-1, hMSH-2, and hMSH-6 protein expression. MSI analysis was performed on those polyps with adequate DNA remaining. Results . 124 HPs were identified 78 sporadic, 21 HNPCC, 25 familial, and the HNPCC group demonstrated no significant differences in overall methylation ( P = .186 Chi 2 ). The familial group demonstrated significantly less over all methylation levels ( P = .004 Chi 2 ). Conclusions . HPs that occur in HNPCC have no more worrying features at a molecular level than those patients with HPs in a sporadic setting.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom