Preparation and Characterization of Water-Soluble Chitosan Microparticles Loaded with Insulin Using the Polyelectrolyte Complexation Method | Zendy

Sihui Wu | Zendy; Tao Yi | Zendy; Hongliang Zhang | Zendy; Zhengquan Su | Zendy

Open Access

Preparation and Characterization of Water-Soluble Chitosan Microparticles Loaded with Insulin Using the Polyelectrolyte Complexation Method

Author(s) -

Sihui Wu,

Tao Yi,

Hongliang Zhang,

Zhengquan Su

Publication year - 2011

Publication title -

journal of nanomaterials

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.463

H-Index - 66

eISSN - 1687-4129

pISSN - 1687-4110

DOI - 10.1155/2011/404523

Subject(s) - chitosan , insulin , polyelectrolyte , insulin delivery , materials science , delivery system , nuclear chemistry , chromatography , chemical engineering , chemistry , biomedical engineering , polymer , medicine , diabetes mellitus , biochemistry , composite material , endocrinology , type 1 diabetes , engineering

Polymeric delivery systems based on microparticles have emerged as a promising approach for peroral insulin delivery. The amount of insulin was quantified by the improved Bradford method. It was shown that water-soluble chitosan/insulin/tripolyphosphate (TPP) mass ratio played an important role in microparticles formation. Stable, uniform, and spherical water-soluble chitosan microparticles (WSC-MPs) with high insulin association efficiency were formed at or close to optimized WSC/insulin/TPP mass ratio. WSC-MPs had higher association efficiency in the pH 4.0 and pH 9.7 of TPP solution. The results showed that association efficiency and loading capacity of insulin-loaded WSC-MPs prepared in 0.01 mol/L HCl of insulin were 48.28 ± 0.90% and 9.52 ± 1.34%. The average size of insulin-loaded WSC-MPs was 292 nm. The presented WSC microparticulate system has promising properties towards the development of an oral delivery system for insulin

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