An Optically Controlled 3D Cell Culturing System | Zendy

K. Ishii | Zendy; Wenqi Hu | Zendy; Swapnil Namekar | Zendy; Aaron T. Ohta | Zendy

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An Optically Controlled 3D Cell Culturing System

Author(s) -

K. Ishii,

Wenqi Hu,

Swapnil Namekar,

Aaron T. Ohta

Publication year - 2011

Publication title -

advances in optoelectronics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.118

H-Index - 21

eISSN - 1687-5648

pISSN - 1687-563X

DOI - 10.1155/2011/253989

Subject(s) - hela , microfluidics , substrate (aquarium) , scaffold , biocompatible material , cell culture , cell , materials science , 3d cell culture , biophysics , viability assay , chemistry , nanotechnology , biomedical engineering , biology , engineering , biochemistry , ecology , genetics

A novel 3D cell culture system was developed and tested. The cell culture device consists of a microfluidic chamber on an optically absorbing substrate. Cells are suspended in a thermoresponsive hydrogel solution, and optical patterns are utilized to heat the solution, producing localized hydrogel formation around cells of interest. The hydrogel traps only the desired cells in place while also serving as a biocompatible scaffold for supporting the cultivation of cells in 3D. This is demonstrated with the trapping of MDCK II and HeLa cells. The light intensity from the optically induced hydrogel formation does not significantly affect cell viability.

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