Cell Uptake and Validation of Novel PECs for Biomedical Applications | Zendy

Ilaria Elena Palamà | Zendy; Mariarosaria Musarò | Zendy; Addolorata M. L. Coluccia | Zendy; Stefania D’Amone | Zendy; Giuseppe Gigli | Zendy

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Cell Uptake and Validation of Novel PECs for Biomedical Applications

Author(s) -

Ilaria Elena Palamà,

Mariarosaria Musarò,

Addolorata M. L. Coluccia,

Stefania D’Amone,

Giuseppe Gigli

Publication year - 2011

Publication title -

journal of drug delivery

Language(s) - English

Resource type - Journals

eISSN - 2090-3014

pISSN - 2090-3022

DOI - 10.1155/2011/203676

Subject(s) - allylamine , polyelectrolyte , fluorescein , trypan blue , fluorescein isothiocyanate , conjugate , hydrochloride , chemistry , viability assay , chromatography , scanning electron microscope , fluorescence microscope , confocal microscopy , fluorescence , biophysics , nanotechnology , cell , materials science , biochemistry , biology , organic chemistry , polymer , mathematical analysis , physics , mathematics , quantum mechanics , composite material , microbiology and biotechnology

This pilot study provides the proof of principle for biomedical application of novel polyelectrolyte complexes (PECs) obtained via electrostatic interactions between dextran sulphate (DXS) and poly(allylamine hydrochloride) (PAH). Scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed that DXS/PAH polyelectrolyte complexes were Monodispersed with regular rounded-shape features and average diameters of 250 nm at 2 : 1 weight ratios of DXS/PAH. Fluorescently labelled DXS and fluorescein-isothiocyanate- (FITC-)conjugate DXS were used to follow cell uptake efficiency of PECs and biodegradability of their enzymatically degradable DXS-layers by using confocal laser scanning microscopy (CLSM). Moreover, quantitative MTT and Trypan Blue assays were employed to validate PECs as feasible and safe nanoscaled carriers at single-cell level without adverse effects on metabolism and viability.

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