Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts fromCarica papayaLeaves | Zendy

Paula Melariri | Zendy; William E. Campbell | Zendy; Paschal Etusim | Zendy; Peter G. Smith | Zendy

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Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts fromCarica papayaLeaves

Author(s) -

Paula Melariri,

William E. Campbell,

Paschal Etusim,

Peter G. Smith

Publication year - 2011

Publication title -

journal of parasitology research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.46

H-Index - 27

eISSN - 2090-0031

pISSN - 2090-0023

DOI - 10.1155/2011/104954

Subject(s) - carica , ethyl acetate , fractionation , chromatography , bioassay , chemistry , high performance liquid chromatography , gas chromatography–mass spectrometry , mass spectrometry , biology , botany , genetics

We investigated the antiplasmodial properties of crude extracts from Carica papaya leaves to trace the activity through bioassay-guided fractionation. The greatest antiplasmodial activity was observed in the ethyl acetate crude extract. C. papaya showed a high selectivity for P. falciparum against CHO cells with a selectivity index of 249.25 and 185.37 in the chloroquine-sensitive D10 and chloroquine-resistant DD2 strains, respectively. Carica papaya ethyl acetate extract was subjected to bioassay-guided fractionation to ascertain the most active fraction, which was purified and identified using high-pressure liquid chromatography (HPLC) and GC-MS (Gas chromatography-Mass spectrometry) methods. Linoleic and linolenic acids identified from the ethyl acetate fraction showed IC 50 of 6.88 μ g/ml and 3.58 μ g/ml, respectively. The study demonstrated greater antiplasmodial activity of the crude ethyl acetate extract of Carica papaya leaves with an IC 50 of 2.96 ± 0.14 μ g/ml when compared to the activity of the fractions and isolated compounds.

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