Comparative Proteomic Analysis of Proteins Involved in the Tumorigenic Process of Seminal Vesicle Carcinoma in Transgenic Mice | Zendy

WeiChao Chang | Zendy; Chuan-Kai Chou | Zendy; ChihChiang Tsou | Zendy; ShengHsiang Li | Zendy; CheinHung Chen | Zendy; Yu-Xing Zhuo | Zendy; Wen−Lian Hsu | Zendy; ChungHsuan Chen | Zendy

Open Access

Comparative Proteomic Analysis of Proteins Involved in the Tumorigenic Process of Seminal Vesicle Carcinoma in Transgenic Mice

Author(s) -

WeiChao Chang,

Chuan-Kai Chou,

ChihChiang Tsou,

ShengHsiang Li,

CheinHung Chen,

Yu-Xing Zhuo,

Wen−Lian Hsu,

ChungHsuan Chen

Publication year - 2010

Publication title -

international journal of proteomics

Language(s) - English

Resource type - Journals

eISSN - 2090-2174

pISSN - 2090-2166

DOI - 10.1155/2010/726968

Subject(s) - seminal vesicle , proteome , western blot , secretion , transgene , genetically modified mouse , microbiology and biotechnology , vesicle , biology , cancer , biochemistry , gene , genetics , prostate , membrane

We studied the seminal vesicle secretion (SVS) of transgenic mice by using one-dimensional gel electrophoresis combined with LTQ-FT ICR MS analysis to explore protein expression profiles. Using unique peptide numbers as a cut-off criterion, 79 proteins were identified with high confidence in the SVS proteome. Label-free quantitative analysis was performed by using the IDEAL_Q software program. Furthermore, western blot assays were performed to validate the expression of seminal vesicle proteins. Sulfhydryl oxidase 1, glia-derived nexin, SVS1, SVS3, and SVS6 showed overexpression in SVS during cancer development. With high sequence similarity to human semenogelin, SVS2 is the most abundance protein in SVS and is dramatically decreased during the tumorigenic process. Our results indicate that these protein candidates could serve as potential targets for monitoring seminal vesicle carcinoma. Moreover, this information can provide clues for investigating seminal vesicle secretion-containing seminal plasma for related human diseases.

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