A Stability Indicating RP-HPLC Method for the Estimation of Gemcitabine HCl in Injectable Dosage forms | Zendy

Mastanamma Shaik | Zendy; G. Ramkumar | Zendy; Deepak Kumar | Zendy; J. V. L. N. Seshagiri Rao | Zendy

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A Stability Indicating RP-HPLC Method for the Estimation of Gemcitabine HCl in Injectable Dosage forms

Author(s) -

Mastanamma Shaik,

G. Ramkumar,

Deepak Kumar,

J. V. L. N. Seshagiri Rao

Publication year - 2010

Publication title -

journal of chemistry

Language(s) - English

Resource type - Journals

eISSN - 2090-9063

pISSN - 2090-9071

DOI - 10.1155/2010/724915

Subject(s) - chemistry , chromatography , gemcitabine , high performance liquid chromatography , dosage form , methanol , forced degradation , phosphate buffered saline , degradation (telecommunications) , reversed phase chromatography , organic chemistry , chemotherapy , surgery , medicine , telecommunications , computer science

A stability indicating RP HPLC method has been developed for the determination of gemcitabine hydrochloride. Chromatography was carried out on an ODS C18 column (250×4.6 mm; 5μ) using a mixture of methanol and phosphate buffer (40: 60 v/v ) as the mobile phase at a flow rate of 1.0 mL/min. The detection of the drug was monitored at 270 nm. The retention time of the drug was found to be 2.31 min. The method produced linear responses in the concentration range of 10 to 60 μg/mL of gemcitabine HCl. The method was found to be reproducible for analysis of the drug in injectable dosage forms. The stability of the drug was assessed by forced degradation studies

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