Open AccessFTIR imaging and ATR-FT-Far-IR synchrotron spectroscopy of pig ear skin
Author(s) -
Tanja M. Greve,
Kristine B. Andersen,
Ole F. Nielsen,
Anders Engdahl
Publication year - 2010
Publication title -
spectroscopy an international journal
Language(s) - English
Resource type - Journals
eISSN - 1875-922X
pISSN - 0712-4813
DOI - 10.1155/2010/716473
Subject(s) - fourier transform infrared spectroscopy , synchrotron radiation , synchrotron , spectroscopy , chemistry , infrared spectroscopy , attenuated total reflection , analytical chemistry (journal) , crystallography , materials science , optics , chromatography , physics , organic chemistry , quantum mechanics
FTIR imaging was performed on pig ear skin samples cryo-sectioned perpendicular to the skin surface. The OH-stretch region revealed the distribution of water; the amide II band gave the protein distribution; the C═O stretch and C–H stretch regions showed the variation in lipids. Water and proteins were similarly distributed. Triglycerides were predominantly found in the deeper skin layers whereas free fatty acids and ceramides were more dominant in the upper layers. ATR-FT-Far-IR spectroscopy with synchrotron radiation was used on full thickness pig ear skin biopsies. The Far-IR spectra showed bands in the region from 100–150 cm –1 due to hydrogen bonded proteins and a band around 180 cm –1 arising from “free” water.
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