Use of Tetra-ammonium Tetrakis(4-Sulphonato)Phenyl Porphyrin forPseudomonasandBacillusCell Imaging | Zendy

V. Sujatha | Zendy; Bharat Sridhar | Zendy; Srinath Krishnamurthy | Zendy; K. S. V. Sampath Kumar | Zendy; K. Senthil Kumar | Zendy; Gautam Pennathur | Zendy

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Use of Tetra-ammonium Tetrakis(4-Sulphonato)Phenyl Porphyrin forPseudomonasandBacillusCell Imaging

Author(s) -

V. Sujatha,

Bharat Sridhar,

Srinath Krishnamurthy,

K. S. V. Sampath Kumar,

K. Senthil Kumar,

Gautam Pennathur

Publication year - 2010

Publication title -

international journal of analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.352

H-Index - 16

eISSN - 1687-8779

pISSN - 1687-8760

DOI - 10.1155/2010/697528

Subject(s) - bacillus cereus , porphyrin , tetra , fluorescence , bacteria , bacillus (shape) , pseudomonas , ammonium acetate , ammonium , chemistry , cereus , fluorescence microscope , nuclear chemistry , microbiology and biotechnology , photochemistry , biology , organic chemistry , medicinal chemistry , optics , physics , genetics , high performance liquid chromatography

The use of tetraammonium tetrakis(4-sulphonato)phenyl porphyrin (TPPS), a water-soluble anionic compound, as a stain to analyse bacterial cells using fluorescent microscopy was investigated. TPPS was effectively used to analyse two different bacteria: Pseudomonas aeruginosa and Bacillus cereus. The variation in brightness with varying concentrations of TPPS was studied. The patterns of variations for these bacteria were found to be the same, but with consistently higher brightness for Bacillus cereus.

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