Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France) | Zendy

M. Biendo | Zendy; Crespin Adjidé | Zendy; Sandrine Castelain | Zendy; Mohamed Belmekki | Zendy; Florence Rousseau | Zendy; M. Slama | Zendy; O. Ganry | Zendy; J.L. Schmit | Zendy; F. Eb | Zendy

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Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France)

Author(s) -

M. Biendo,

Crespin Adjidé,

Sandrine Castelain,

Mohamed Belmekki,

Florence Rousseau,

M. Slama,

O. Ganry,

J.L. Schmit,

F. Eb

Publication year - 2010

Publication title -

international journal of microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.696

H-Index - 40

eISSN - 1687-9198

pISSN - 1687-918X

DOI - 10.1155/2010/150464

Subject(s) - teicoplanin , multilocus sequence typing , pulsed field gel electrophoresis , glycopeptide , enterococcus faecium , microbiology and biotechnology , biology , vancomycin , enterococcus faecalis , enterococcus , gene , glycopeptide antibiotic , genetics , genotype , escherichia coli , bacteria , antibiotics , staphylococcus aureus

We studied 138 glycopeptide-resistant enterococci (GRE) strains, consisting of 131 glycopeptide-resistant Enterococcus faecium (GREfm) and 7 glycopeptide-resistant Enterococcus faecalis (GREfs). The GREfm strains were resistant to penicillin, ampicillin, vancomycin, and teicoplanin, while the GREfs strains were only resistant to vancomycin and teicoplanin. The van A gene was the only glycopeptide determinant present in all GRE isolates investigated. Genes coding for Hyl and Hyl+ Esp were detected in 39 (29.8%) and 92 (70.2%) of the 131 GREfm isolates, respectively. Three of the 7 GREfs were positive for gelE + asa 1 genes, 3 for gel E gene, and 1 for asa 1 gene. The genetic relationship between the 138 GRE was analyzed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). GREfm isolates were clustered in a single genogroup (pulsotype A), and GREfs were clustered in six genogroups (pulsotypes B-G). Among the isolates investigated by MLST, only 18 PCR products were sequenced (12 E. faecium and 6 E. faecalis ), and 9 sequence types (STs) were identified.

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