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Stoichiometry and equilibrium study of ion-ligand conducted in aqueous solution by spectrophotometry. Stability constants of the complexes are determined at 25 o C, ionic strength was adjusted to 0.2 (% w/v) with NaCl. The pH was adjusted to 6. 2-Thiobarbituric acid-ion complexes' stability was calculated in aqueous solution using DATAN (Data Analysis program). The results of this method denote formation specie ML 2. The log K for ions Cu +2 ,Co +2 ,Ni +2 and Zn +2 were 4.89, 4.69, 4.47 and 4.82, respectively.

Spectrophotometric Evaluation of Stability Constants of Copper, Cobalt, Nickel and Zinc with 2-Thiobarbituric Acid in Aqueous Solution