Open AccessTyrosinase‐Immobilized Biosensor Based on the Functionalized Hydroxyl Group‐MWNT and Detection of Phenolic Compounds in Red Wines
Author(s) -
Jihoon Yang,
Jae-Chan Lee,
SeongHo Choi
Publication year - 2009
Publication title -
journal of sensors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.399
H-Index - 43
eISSN - 1687-7268
pISSN - 1687-725X
DOI - 10.1155/2009/916515
Subject(s) - biosensor , tyrosinase , phenol , chemistry , carbon nanotube , phenols , phosphate buffered saline , polymerization , chromatography , nuclear chemistry , organic chemistry , polymer , materials science , nanotechnology , enzyme , biochemistry
The tyrosinase-immobilized biosensor was developed with the hydroxyl group-functionalized multiwall carbon nanotube (MWNT) for phenol detection. The hydroxyl group-modified MWNT was modified to include poly(GVPB)-g-MWNT, or poly(HEMA), by a radiation-induced graft polymerization of glucosyl 4-vinylphenylboronate (GVPB) or 2-hydroxyethyl methacrylate (HEMA) on the surface of MWNT. The response of biosensor was in the range of 0.6–7.0 mM for concentration and in the range of 0.05–0.35 mM for phenol in a phosphate buffer solution, respectively. Various parameters influencing biosensor performance have been optimized: for pH, temperature, and the response to various phenolic compounds. The biosensor was then tested on phenolic compounds contained in three different commercial red wines
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