Persistently Elevated Level of IL-8 inChlamydia trachomatisInfected HeLa 229 Cells is Dependent on Intracellular Available Iron | Zendy

Harsh Vardhan | Zendy; Raini Dutta | Zendy; Vikas Vats | Zendy; Rishein Gupta | Zendy; Rajneesh Jha | Zendy; Hem Chandra Jha | Zendy; Pragya Srivastava | Zendy; Apurb Rashmi Bhengraj | Zendy; Aruna Mittal | Zendy

Open Access

Persistently Elevated Level of IL-8 inChlamydia trachomatisInfected HeLa 229 Cells is Dependent on Intracellular Available Iron

Author(s) -

Harsh Vardhan,

Raini Dutta,

Vikas Vats,

Rishein Gupta,

Rajneesh Jha,

Hem Chandra Jha,

Pragya Srivastava,

Apurb Rashmi Bhengraj,

Aruna Mittal

Publication year - 2009

Publication title -

mediators of inflammation

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.37

H-Index - 97

eISSN - 1466-1861

pISSN - 0962-9351

DOI - 10.1155/2009/417658

Subject(s) - chlamydia trachomatis , proinflammatory cytokine , chlamydia , secretion , hela , biology , chemokine , immunology , tumor necrosis factor alpha , intracellular , interleukin 6 , microbiology and biotechnology , virology , inflammation , cell , endocrinology , genetics

Chlamydia trachomatis is a leading cause of sexually transmitted infection worldwide and responsible for myriad of immunopathological changes associated with reproductive health. Delayed secretion of proinflammatory chemokine interleukin (IL)-8 is a hallmark of chlamydial infection and is dependent on chlamydial growth. We examined the effect of iron chelators on IL-8 production in HeLa 229 (cervix epitheloid cell, CCL2) cells infected with C. trachomatis . IL-8 production was induced by Iron chelator DFO and Mimosine, however, synergy with chlamydial infection was obtained with DFO only. Temporal expression of proinflammatory secreted cytokines IL-1beta, TNF-alpha, and IL-8 did not show synchrony in Chlamydia trachomatis infected cells. Secretion of IL-8 from Hela cells infected with C. trachomatis was not dependent on IL-1 beta and TNF- alpha induction. These results indicate towards involvement of iron in chlamydia induced IL-8 production.

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