Secretory Leukoprotease Inhibitor: A Native Antimicrobial Protein in the Innate Immune Response in a Rat Model ofS. aureusKeratitis | Zendy

Víctor E. Reviglio | Zendy; Andrés R. Grenat | Zendy; Federico Pegoraro | Zendy; Rubén Sambuelli | Zendy; Tayyib S. Rana | Zendy; Irene C. Kuo | Zendy

Open Access

Secretory Leukoprotease Inhibitor: A Native Antimicrobial Protein in the Innate Immune Response in a Rat Model ofS. aureusKeratitis

Author(s) -

Víctor E. Reviglio,

Andrés R. Grenat,

Federico Pegoraro,

Rubén Sambuelli,

Tayyib S. Rana,

Irene C. Kuo

Publication year - 2009

Publication title -

journal of ophthalmology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.818

H-Index - 40

eISSN - 2090-0058

pISSN - 2090-004X

DOI - 10.1155/2009/259393

Subject(s) - slpi , medicine , innate immune system , proinflammatory cytokine , keratitis , antimicrobial peptides , immunohistochemistry , antimicrobial , immunology , staphylococcus aureus , immune system , tumor necrosis factor alpha , microbiology and biotechnology , beta defensin , matrix metalloproteinase , fungal keratitis , inflammation , biology , dermatology , bacteria , genetics

Purpose . To describe the presence of secretory leukocyte protease inhibitor (SLPI), a cationic peptide with antimicrobial and antiprotease activity in the innate immune reaction in a rat model of Staphylococcus aureus keratitis. Methods . Forty female Lewis rats were divided into 2 groups: the infectious keratitis and the epithelial defect groups. Eyes were processed for immunohistochemical studies for SLPI, interleukin-1, interleukin-6, tumor necrosis factor-alpha, and matrix metalloproteinase-8. Results . Immunohistochemical studies confirmed high levels of SLPI, IL-1, IL-6, TNF- α , and MMP-8 expression in eyes with S. aureus keratitis and with epithelial defects, in contrast to undetectable SLPI expression in the normal control corneas. Conclusions . To our knowledge, this paper is the first to demonstrate the presence of SLPI with increased amounts of proinflammatory cytokines in inflamed and infected corneas.

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