Open AccessDevelopment and Validation of RP‐HPLC Method for the Determination of Adefovir Dipivoxil in Bulk and in Pharmaceutical Formulation
Author(s) -
Ahmed Zaheer,
B. Gopinath,
A. Sathish Kumar Shetty,
B. K. Sridhar
Publication year - 2008
Publication title -
journal of chemistry
Language(s) - English
Resource type - Journals
eISSN - 2090-9071
pISSN - 2090-9063
DOI - 10.1155/2009/157868
Subject(s) - adefovir , chromatography , acetonitrile , phosphate buffered saline , linearity , selectivity , high performance liquid chromatography , chemistry , detection limit , pharmaceutical formulation , virology , biochemistry , biology , virus , quantum mechanics , physics , lamivudine , catalysis , hepatitis b virus
A rapid and sensitive RP-HPLC method with UV detection (262 nm) for routine analysis of adefovir dipivoxil in bulk and in pharmaceutical formulation was developed. Chromatography was performed with mobile phase containing a mixture of acetonitrile and phosphate buffer (50:50, v/v) with flow rate 1.0 mL min-l. In the range of 5.0-100 µg/mL, the linearity of adefovir dipivoxil shows a correlation co-efficient of 0.9999. The proposed method was validated by determining sensitivity accuracy, precision, robustness stability, specificity, selectivity and system suitability parameters
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