Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling | Zendy

Grace O’Maille | Zendy; Eden P. Go | Zendy; Linh Hoang | Zendy; Elizabeth J. Want | Zendy; Colin A. Smith | Zendy; Paul E. O’Maille | Zendy; Anders Nordström | Zendy; Hirotoshi Morita | Zendy; Chuan Qin | Zendy; Wilasinee Uritboonthai | Zendy; Junefredo V. Apon | Zendy; Richard O. Moore | Zendy; James M. Garrett | Zendy; Gary Siuzdak | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling

Author(s) -

Grace O’Maille,

Eden P. Go,

Linh Hoang,

Elizabeth J. Want,

Colin A. Smith,

Paul E. O’Maille,

Anders Nordström,

Hirotoshi Morita,

Chuan Qin,

Wilasinee Uritboonthai,

Junefredo V. Apon,

Richard O. Moore,

James M. Garrett,

Gary Siuzdak

Publication year - 2008

Publication title -

spectroscopy an international journal

Language(s) - English

Resource type - Journals

eISSN - 1875-922X

pISSN - 0712-4813

DOI - 10.1155/2008/715905

Subject(s) - derivatization , chromatography , chemistry , metabolite , metabolomics , mass spectrometry , chemical ionization , reagent , atmospheric pressure chemical ionization , ionization , ion , organic chemistry , biochemistry

Comprehensive detection and quantitation of metabolites from a biological source constitute the major challenges of current metabolomics research. Two chemical derivatization methodologies, butylation and amination, were applied to human serum for ionization enhancement of a broad spectrum of metabolite classes, including steroids and amino acids. LC-ESI-MS analysis of the derivatized serum samples provided a significant signal elevation across the total ion chromatogram to over a 100-fold increase in ionization efficiency. It was also demonstrated that derivatization combined with isotopically labeled reagents facilitated the relative quantitation of derivatized metabolites from individual as well as pooled samples.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research