Association of Single Nucleotide Polymorphisms in the IL‐18 Gene with Production of IL‐18 Protein by Mononuclear Cells from Healthy Donors | Zendy

Olga P. Khripko | Zendy; Sennikova Nadegda Sergeevna | Zendy; Julia Lopatnikova | Zendy; Khripko Jurii Ivanovich | Zendy; Filipenko Maxim Leonidovich | Zendy; Khrapov Evgenii Alexandrovich | Zendy; Gelfgat Evgenii L′vovich | Zendy; Yakushenko Elena Vladimirovna | Zendy; Kozlov Va | Zendy; С. В. Сенников | Zendy

Open Access

Association of Single Nucleotide Polymorphisms in the IL‐18 Gene with Production of IL‐18 Protein by Mononuclear Cells from Healthy Donors

Author(s) -

Olga P. Khripko,

Sennikova Nadegda Sergeevna,

Julia Lopatnikova,

Khripko Jurii Ivanovich,

Filipenko Maxim Leonidovich,

Khrapov Evgenii Alexandrovich,

Gelfgat Evgenii L′vovich,

Yakushenko Elena Vladimirovna,

Kozlov Va,

С. В. Сенников

Publication year - 2008

Publication title -

mediators of inflammation

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.37

H-Index - 97

eISSN - 1466-1861

pISSN - 0962-9351

DOI - 10.1155/2008/309721

Subject(s) - single nucleotide polymorphism , peripheral blood mononuclear cell , allele , biology , gene , promoter , proinflammatory cytokine , microbiology and biotechnology , immunology , gene expression , genotype , genetics , inflammation , in vitro

IL-18 has proinflammatory effects and participates in both innate and adaptive cellular and humoral immunity. A number of SNPs that influence IL-18 production are found in the gene promoter region. We investigated the association of SNPs in the IL-18 promoter at − 607 and − 137 with the level of IL-18 protein production by PBMC from healthy donors from Southwestern Siberia. The genetic distribution of these SNPs in the promoter site was established by PCR. IL-18 protein production was determined by ELISA. Our results showed that PBMC from donors carrying allele 137C have lower levels of both spontaneous and LPS-stimulated IL-18 production. In contrast, PBMC from donors carrying allele 607A showed significant increases in spontaneous and stimulated IL-18 production compared to wild type. Our study suggests that the SNPs − 607 and − 137 in the promoter region of the IL-18 gene influence the level of IL-18 protein production by PBMC from healthy donors in Southwestern Siberia.

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