Nanotomography of Cell Surfaces with Evanescent Fields | Zendy

Michael Wagner | Zendy; Petra Weber | Zendy; Wolfgang S. L. Strauß | Zendy; HenriPierre Lassalle | Zendy; Herbert Schneckenburger | Zendy

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Nanotomography of Cell Surfaces with Evanescent Fields

Author(s) -

Michael Wagner,

Petra Weber,

Wolfgang S. L. Strauß,

HenriPierre Lassalle,

Herbert Schneckenburger

Publication year - 2008

Publication title -

advances in optical technologies

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.124

H-Index - 25

eISSN - 1687-6407

pISSN - 1687-6393

DOI - 10.1155/2008/254317

Subject(s) - total internal reflection fluorescence microscope , substrate (aquarium) , nanometre , topology (electrical circuits) , optics , materials science , reflection (computer programming) , fluorescence microscope , resolution (logic) , metaphase , microscopy , fluorescence , nanotechnology , biophysics , optoelectronics , physics , chemistry , computer science , biology , mathematics , ecology , biochemistry , chromosome , combinatorics , artificial intelligence , gene , programming language

The technique of variable-angle total internal reflection fluorescence microscopy (TIRFM) and its application to nanotomography of cell surfaces are described. Present applications include (1) 3D imaging of chromosomes in their metaphase to demonstrate axial resolution in the nanometre range, (2) measurements of cell-substrate topology, which upon cholesterol depletion shows some loosening of cell-substrate contacts, and (3) measurements of cell topology upon photodynamic therapy (PDT), which demonstrate cell swelling and maintenance of focal contacts. The potential of the method for in vitro diagnostics, but also some requirements and limitations are discussed

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