Pigment Melanin Scavenges Nitric Oxide In Vitro: Possible Relevance to Keloid Formation | Zendy

Julian M. Menter | Zendy; Danita Eatman | Zendy; Mohamed A. Bayorh | Zendy; Ahmad M. Dawaghreh | Zendy; Isaac Willis | Zendy

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Pigment Melanin Scavenges Nitric Oxide In Vitro: Possible Relevance to Keloid Formation

Author(s) -

Julian M. Menter,

Danita Eatman,

Mohamed A. Bayorh,

Ahmad M. Dawaghreh,

Isaac Willis

Publication year - 2008

Publication title -

research letters in physical chemistry

Language(s) - English

Resource type - Journals

eISSN - 1687-6881

pISSN - 1687-6873

DOI - 10.1155/2008/210616

Subject(s) - sepia , nitric oxide , chemistry , melanin , cuttlefish , substrate (aquarium) , pigment , in vitro , biophysics , adsorption , biochemistry , organic chemistry , food science , biology , botany , officinalis , ecology

Recently, nitric oxide (NO) has been implicated in the formation of keloids, preferentially formed in dark-skinned persons, and we suspected that pigment melanin itself may play a direct role by adsorbing NO. We tested the ability of cuttlefish sepia melanin to scavenge (adsorb) NO, generated in situ by 2-(N.N Diethylamino) diazeneolate-2-oxide (DEA/NO), through a dialysis membrane. NO was measured as NO2_ and NO3_ by the Griess method and as N2O3 by trapping experiments with the fluorogenic substrate 4,5-diaminofluorescein (DAF-2). Initial NO2_ and NO3_ concentrations were significantly lower in the test dialyzates than in controls. Scavenging of NO was rapid enough to compete with DAF adduct formation. Both analytical methods gave comparable results. Adsorbed NO and/or its oxidized products may undergo interactions with melanin, adsorbed O2, and/or dermal material that may lead to keloid formation

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