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Aim.  The aim of this study was to determine tumor necrosis factor-alpha (TNF-    α    ) levels in periapical exudates and to evaluate their relationship with radiological findings.   Methodology.  Periapical exudates were collected from root canals of 60 single-rooted teeth using absorbent paper points.  TNF-    αlevels were determined by enzyme-linked immunosorbent assays. The samples were divided into three groups according to the periapical radiolucent area.   Results.  Nonparametric Kruskal-Wallis test revealed significant differences between  TNF-    αconcentrations in control group (40,57  ±  28    , 15 pg/mL) and group with larger radiolucent areas (2365,79  ±  582    , 95 pg/mL), as well as between control and canals with small radiolucent areas  (507,66  ±  278    , 97) (    P  <  .05    ).  Conclusions.  The levels of  TNF-    αincrease significantly in teeth with periapical pathosis, from smaller to bigger lesions. This research and its results have shown that objective analysis of the  TNF-    αlevels enables establishment of a relationship between different concentrations of TNF-    αand different radiological changes.

Tumor Necrosis Factor-Alpha in Peripical Tissue Exudates of Teeth with Apical Periodontitis