Optimization of Protease Production fromAspergillus OryzaeSp. Using Box-Behnken Experimental Design | Zendy

G. Srinu Babu | Zendy; Rayaprolu Kiran | Zendy; N. Lokeswari | Zendy; K. Jaya Raju | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Optimization of Protease Production fromAspergillus OryzaeSp. Using Box-Behnken Experimental Design

Author(s) -

G. Srinu Babu,

Rayaprolu Kiran,

N. Lokeswari,

K. Jaya Raju

Publication year - 2006

Publication title -

journal of chemistry

Language(s) - English

Resource type - Journals

eISSN - 2090-9063

pISSN - 2090-9071

DOI - 10.1155/2007/254613

Subject(s) - aspergillus oryzae , box–behnken design , response surface methodology , protease , alkaline protease , central composite design , chemistry , enzyme , chromatography , food science , microbiology and biotechnology , biochemistry , biology

Protease production by Aspergillus oryzae was optimized in shake- flask cultures using Box-Behnken experimental design. An empirical model was developed through response surface methodology to describe the relationship between tested variable (peptone, glucose, soyabeanmeal and pH). Maximum enzyme activity was attained with Peptone at 4 g/L; temperature at 30 0C glucose at 6 g/L; 30 0C and pH at 10. Experimental verification of the model showed a validation of 95%, which is more than 3-fold increase compare to the basal medium

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research