Open AccessImplementation of Accurate and Fast DNA Cytometry by Confocal Microscopy in 3D
Author(s) -
Lennert S. Ploeger,
André Huisman,
Jurryt van der Gugten,
Dionne M. van der Giezen,
Jeroen A.M. Beliën,
Abdelhadi Y. Abbaker,
Hub F. J. Dullens,
William E. Grizzle,
Neal Poulin,
Gerrit A. Meijer,
P. J. van Diest
Publication year - 2005
Publication title -
analytical cellular pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 24
eISSN - 2210-7185
pISSN - 2210-7177
DOI - 10.1155/2005/289216
Subject(s) - cytometry , context (archaeology) , confocal , microscopy , biomedical engineering , confocal microscopy , dna , microscope , computer science , materials science , chemistry , flow cytometry , biology , microbiology and biotechnology , pathology , physics , optics , medicine , biochemistry , paleontology
DNA cytometry is a powerful method for measuring genomic instability. Standard approaches that measure DNA content of isolated cells may induce selection bias and do not allow interpretation of genomic instability in the context of the tissue. Confocal Laser Scanning Microscopy (CLSM) provides the opportunity to perform 3D DNA content measurements on intact cells in thick histological sections. Because the technique is technically challenging and time consuming, only a small number of usually manually selected nuclei were analyzed in different studies, not allowing wide clinical evaluation. The aim of this study was to describe the conditions for accurate and fast 3D CLSM cytometry with a minimum of user interaction to arrive at sufficient throughput for pilot clinical applications.
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