Subcellular localisation of cholesterol and phosphocholine with pattern-recognition-imaging-TOF-SIMS | Zendy

Per Malmberg | Zendy; Håkan Nygren | Zendy; Peter Sjövall | Zendy; Jukka Lausmaa | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Subcellular localisation of cholesterol and phosphocholine with pattern-recognition-imaging-TOF-SIMS

Author(s) -

Per Malmberg,

Håkan Nygren,

Peter Sjövall,

Jukka Lausmaa

Publication year - 2004

Publication title -

journal of spectroscopy

Language(s) - English

Resource type - Journals

eISSN - 2314-4920

pISSN - 2314-4939

DOI - 10.1155/2004/928263

Subject(s) - phosphocholine , chemistry , ion , resolution (logic) , secondary ion mass spectrometry , mass spectrum , mass spectrometry , membrane , analytical chemistry (journal) , phosphatidylcholine , cholesterol , phospholipid , cell membrane , high resolution , biophysics , chromatography , biochemistry , biology , remote sensing , organic chemistry , artificial intelligence , computer science , geology

Molecular ions of cholesterol, and its fragments, and phosphocholine fragments of phospholipids, were localized in single cells with a resolution of 7000 or high lateral resolution. High mass resolution spectra were recorded on reference samples of pure cholesterol and phosphatidylcholine. Characteristic fragment peaks and the silver cationised quasimolecular ion (M+Ag) + were selected as a pattern for the identification of the lipids in TOF-SIMS images of surface-adhering leukocytes. The localisation of membrane lipids showed lateral heterogeneity over the cell surface.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research