Confocal 3D DNA Cytometry: Assessment of Required Coefficient of Variation by Computer Simulation | Zendy

Lennert S. Ploeger | Zendy; Jeroen A.M. Beliën | Zendy; Neal Poulin | Zendy; William E. Grizzle | Zendy; P. J. van Diest | Zendy

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Confocal 3D DNA Cytometry: Assessment of Required Coefficient of Variation by Computer Simulation

Author(s) -

Lennert S. Ploeger,

Jeroen A.M. Beliën,

Neal Poulin,

William E. Grizzle,

P. J. van Diest

Publication year - 2004

Publication title -

analytical cellular pathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.576

H-Index - 24

eISSN - 2210-7185

pISSN - 2210-7177

DOI - 10.1155/2004/350752

Subject(s) - histogram , sample (material) , coefficient of variation , sample size determination , biological system , dna , cytometry , sample preparation , biology , statistics , materials science , computer science , chemistry , mathematics , flow cytometry , artificial intelligence , chromatography , genetics , image (mathematics)

Confocal Laser Scanning Microscopy (CLSM) provides the opportunity to perform 3D DNA content measurements on intact cells in thick histological sections. So far, sample size has been limited by the time consuming nature of the technology. Since the power of DNA histograms to resolve different stemlines depends on both the sample size and the coefficient of variation (CV) of histogram peaks, interpretation of 3D CLSM DNA histograms might be hampered by both a small sample size and a large CV. The aim of this study was to analyze the required CV for 3D CLSM DNA histograms given a realistic sample size.

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