Total body water and extracellular water measurements through in vivo dilution of D2O and bromide as tracers | Zendy

Giuseppe Sergi | Zendy; Roberta Bertani | Zendy; Irene Calliari | Zendy; Lucia Lupoli | Zendy; Adriano Berton | Zendy; Stefania Volpato | Zendy; Giuliano Enzi | Zendy

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Total body water and extracellular water measurements through in vivo dilution of D₂O and bromide as tracers

Author(s) -

Giuseppe Sergi,

Roberta Bertani,

Irene Calliari,

Lucia Lupoli,

Adriano Berton,

Stefania Volpato,

Giuliano Enzi

Publication year - 2003

Publication title -

journal of spectroscopy

Language(s) - English

Resource type - Journals

eISSN - 2314-4920

pISSN - 2314-4939

DOI - 10.1155/2003/506858

Subject(s) - dilution , bromide , in vivo , extracellular fluid , body water , chemistry , extracellular , environmental chemistry , analytical chemistry (journal) , chromatography , inorganic chemistry , thermodynamics , medicine , physics , body weight , biochemistry , biology , microbiology and biotechnology

Large interest is into the determination of body fluids because of changes in body water in a wide variety of physio- logical and pathological conditions. Here methods based on dilution of tracers, are reported, for the extracellular water (ECW) and total body water (TBW) determinations on the same sample. As for ECW, bromide is used as tracer and its concentration is determined by energy dispersive X-ray spectrometry; while as for TBW, D2O is used as tracer and HOD is determined by FT IR. Both methods represent significant improvements with respect the procedures described in the literature in terms of availability, reproducibility and accuracy.

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