DNA Extraction from Bronchial Aspirates for Molecular Cytology: Which Method to Take? | Zendy

Hans Jürgen Grote | Zendy; Viola Schmiemann | Zendy; Mario Sarbia | Zendy; Alfred Böcking | Zendy

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DNA Extraction from Bronchial Aspirates for Molecular Cytology: Which Method to Take?

Author(s) -

Hans Jürgen Grote,

Viola Schmiemann,

Mario Sarbia,

Alfred Böcking

Publication year - 2002

Publication title -

analytical cellular pathology

Language(s) - English

Resource type - Journals

eISSN - 2210-7185

pISSN - 2210-7177

DOI - 10.1155/2003/354796

Subject(s) - fixative , dna extraction , dna , biology , polymerase chain reaction , genomic dna , dna fragmentation , microbiology and biotechnology , chromatography , chemistry , gene , biochemistry , genetics , staining , apoptosis , programmed cell death

To date, there are only few systematic reports on the quality of DNA extracted from routine diagnostic cytologic specimens. It was the aim of the present study to evaluate the ability of 50% ethanol/2% carbowax (Saccomanno fixative) to preserve bronchial secretions with high quality genomic DNA as well as to compare different DNA extraction methods.

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