High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA1 | Zendy

Jacob Larsen | Zendy; Anne Marie Ottesen | Zendy; Maria Kirchhoff | Zendy; Claes Lundsteen | Zendy; Jørgen K. Larsen | Zendy

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High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA¹

Author(s) -

Jacob Larsen,

Anne Marie Ottesen,

Maria Kirchhoff,

Claes Lundsteen,

Jørgen K. Larsen

Publication year - 2001

Publication title -

analytical cellular pathology

Language(s) - English

Resource type - Journals

eISSN - 2210-7185

pISSN - 2210-7177

DOI - 10.1155/2001/301570

Subject(s) - comparative genomic hybridization , microbiology and biotechnology , genomic dna , dna , biology , oligonucleotide , nucleic acid thermodynamics , genetics , gene , genome , base sequence

We investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP-PCR) as opposed to the use of unamplified DNA. Five DNA samples from B-cell leukemias with small 11q deletions were amplified by DOP-PCR and analysed by means of high resolution comparative genomic hybridization (HR-CGH) for the evaluation of aberration size detection limit. By means of HR-CGH, we found the detection limit of DOP-PCR CGH for deletions to be between 3 Mbp and 7-8 Mbp.

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