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In order to prepare the monoclonal antibody against 1,5-anhydro-glucitol (1), it was conjugated with bovine serum albumin (BSA), human serum albumin (HSA) or chicken lysozyme (CL) using succinate or-alanine succinate as a spacer to produce individual antigen conjugates. The number of hapten contained in each antigen conjugate was determined by matrix- assisted laser desorption/ionization tof mass (MALDI-tof-MS) spectrometry. The formation of monoclonal antibody (MAb) was also discussed.

spectroscopy

Determination of 1,5‒anhydro‒glucitol–carrier protein conjugates by matrix‒assisted laser desorption/ionization tof mass spectrometry and antibody formation