Determination of 1,5‒anhydro‒glucitol–carrier protein conjugates by matrix‒assisted laser desorption/ionization tof mass spectrometry and antibody formation | Zendy

LiJiang Xuan | Zendy; Hiroyuki Tanaka | Zendy; Satoshi Morimoto | Zendy; Yukihiro Shoyama | Zendy; Hiroshi Akanuma | Zendy; K. MURAOKA | Zendy

Open Access

Determination of 1,5‒anhydro‒glucitol–carrier protein conjugates by matrix‒assisted laser desorption/ionization tof mass spectrometry and antibody formation

Author(s) -

LiJiang Xuan,

Hiroyuki Tanaka,

Satoshi Morimoto,

Yukihiro Shoyama,

Hiroshi Akanuma,

K. MURAOKA

Publication year - 2000

Publication title -

journal of spectroscopy

Language(s) - English

Resource type - Journals

eISSN - 2314-4920

pISSN - 2314-4939

DOI - 10.1155/2000/151704

Subject(s) - chemistry , lysozyme , conjugate , mass spectrometry , bovine serum albumin , matrix assisted laser desorption/ionization , hapten , chromatography , conjugated system , monoclonal antibody , serum albumin , desorption , antigen , antibody , biochemistry , organic chemistry , biology , immunology , mathematics , adsorption , polymer , mathematical analysis

In order to prepare the monoclonal antibody against 1,5-anhydro-glucitol (1), it was conjugated with bovine serum albumin (BSA), human serum albumin (HSA) or chicken lysozyme (CL) using succinate or-alanine succinate as a spacer to produce individual antigen conjugates. The number of hapten contained in each antigen conjugate was determined by matrix- assisted laser desorption/ionization tof mass (MALDI-tof-MS) spectrometry. The formation of monoclonal antibody (MAb) was also discussed.

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