Time-Resolved Resonance Raman Studies of Radicals From 4-Aminoresorcinol as Models for the Active Site Radical Intermediate in Copper Amine Oxidases | Zendy

Roger H. Bisby | Zendy; Steve A. Johnson | Zendy; Anthony W. Parker | Zendy; Susan M. Tavender | Zendy

Open Access

Time-Resolved Resonance Raman Studies of Radicals From 4-Aminoresorcinol as Models for the Active Site Radical Intermediate in Copper Amine Oxidases

Author(s) -

Roger H. Bisby,

Steve A. Johnson,

Anthony W. Parker,

Susan M. Tavender

Publication year - 1999

Publication title -

laser chemistry

Language(s) - English

Resource type - Journals

eISSN - 1026-8014

pISSN - 0278-6273

DOI - 10.1155/1999/62680

Subject(s) - chemistry , deprotonation , resonance raman spectroscopy , radiolysis , radical , photochemistry , amine gas treating , raman spectroscopy , resonance (particle physics) , reaction intermediate , organic chemistry , catalysis , ion , physics , particle physics , optics

Radicals formed by one-electron oxidation of 4-aminoresorcinol have been studied as models for the active site free radical intermediate which forms as a stable product during anaerobic incubation of amine oxidases with amine substrates. Pulse radiolysis shows that the radical undergoes ionisations with pKa's of 3.4 and 6.4. Although the two deprotonated forms are difficult to distinguish by absorption spectroscopy, they have clearly different resonance Raman spectra. Comparison with the resonance Raman spectrum of the enzyme intermediate shows it to be the singly deprotonated form of the radical.

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