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The leaf oils of Piper callosum from Peruvian Amazon and Michelia montana from Assam, India, were prepared by hydrodistillation and analyzed by a combination of GC and GC/MS. Twenty five and thirty components have been identified, representing 96.3 and 100.0% of the respective oils. The major constituents were found to be asaricin (syn. sarisan) (35.9 and 81.8%, respectively) and safrole (20.2 and 13.0%). The oil of P. callosum contained in addition eugenyl methyl ether (9.7%) and (E)‒asarone (7.8%), compounds not detected in the M. montana oil. The identity of the principal compound, an isomer of myristicin, was unequivocally established by 13C‒NMR spectrometric techniques, especially long‒range 1H–13C correlation.

Compositional analysis of the leaf oils ofPiper callosumRuiz & Pav. from Peru andMichelia montanaBlume from India